Rat Soluble Intercellular Adhe

Rat Soluble Intercellular Adhesion Molecule 1 ELISA Kit
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Soluble Intercellular adhesion molecule 1(sICAM-1), Soluble Major group rhinovirus receptor, sICAM, Soluble CD54; Soluble rhinovirus
receptor; sBB2; sP3.58; Soluble cell surface glycoprotein P3.58; MALA-2,MyD10,;

Search name
Rat sICAM-1 ELISA Kit, Rat Soluble Intercellular adhesion molecule 1 ELISA Kit, Rat Soluble Major group rhinovirus receptor
ELISA Kit, Rat sICAM ELISA Kit, Rat Soluble CD54 ELISA Kit, Rat Soluble rhinovirus receptor ELISA Kit, Rat sBB2 ELISA Kit, Rat
sP3.58 ELISA Kit, Rat Soluble cell surface glycoprotein P3.58 ELISA Kit, Rat Soluble MALA-2 ELISA Kit, Rat Soluble MyD10
ELISA Kit

Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat SICAM-1 concentrations in serum, Plasma, Urine, tissue
homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to SICAM-1. Standards or samples are then added
to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for SICAM-1 and Avidin
conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to
each well. Only those wells that contain SICAM-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color.
The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of SICAM-1 in the samples is then determined by comparing
the O.D. of the samples to the standard curve.