Rat Soluble Mesothelin Related

Rat Soluble Mesothelin Related Peptide Kit ELISA Kit
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Mesothelin,;CAK1 antigen,Pre-pro-megakaryocyte-potentiating factor, MPF; SMRP; CAK1 antigen; megakaryocyte potentiating factor;
pre-pro-megakaryocyte-potentiating factor; soluble MPF mesothelin related protein

Search name
Rat Mesothelin ELISA Kit, Rat MPF ELISA Kit, Mesothelin ELISA Kit, MPF ELISA Kit, MSLN ELISA KIT, Rat soluble
MPF mesothelin related protein ELISA KIT , Rat SMRP ELISA KIT

Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat Mesothelin concentrations in serum, Plasma, Urine,
tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mesothelin. Standards or samples are then added
to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Mesothelin and Avidin
conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to
each well. Only those wells that contain Mesothelin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in
color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mesothelin in the samples is then determined by
comparing the O.D. of the samples to the standard curve.