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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测牛血清,血浆等样本中目标蛋白的浓度
- 适应物种:
牛
- 标记物:
Bovine Fibronectin,FN
- 样本:
牛的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Bovine Fibronectin, FN ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Fibronectin(FN), fibronectin,fibronectin 1; CIG; ED-B; FINC; FNZ; GFND; GFND2; LETS; MSF; cold-insoluble globulin;
migration-stimulating factor
Search name
Bovine Fibronectin ELISA Kit, Bovine FN ELISA Kit, Bovine fibronectin 1 ELISA Kit, Bovine CIG ELISA Kit, Bovine ED-B ELISA
Kit, Bovine FINC ELISA Kit, Bovine FNZ ELISA Kit, Bovine GFND ELISA Kit, Bovine GFND2 ELISA Kit, Bovine LETS ELISA Kit,
Bovine MSF ELISA Kit, Bovine cold-insoluble globulin ELISA Kit, Bovine migration-stimulating factor ELISA Kit
Intended use
This immunoassay kit allows for the in vitro quantitative determination of bovine fibronectin,FN concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to FN. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for FN and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain FN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of FN in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
cathrynyy 请问各位大侠: 我想做Fn铺板的细胞粘附实验,可是查了半天,发现有好多种,究竟用哪种型号的?哪家公司又便宜,又好用呢? 谢谢! freecell Fn=纤维连接蛋白 ? 究竟哪种好用,取决于所在实验室条件。同样的东西,在不同的实验室有不同的结果。 一般用这个: Fibronectin, bovine plasma (Sigma, cat. no. F1141
TUNEL PROCEDURE IN BOVINE EMBRYOS
. Dilute 1 µl RNase A (stock) with 999 µl PBS/PVP and then dilute this solution 1:1 with PBS/PVP to obtain a working solution (Use fresh). Mounting medium and antifade: ProLong Antifade Kit (Molecular
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