Bovine Fibronectin,FN ELISA Ki

Bovine Fibronectin, FN ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Fibronectin(FN), fibronectin,fibronectin 1; CIG; ED-B; FINC; FNZ; GFND; GFND2; LETS; MSF; cold-insoluble globulin;

migration-stimulating factor

Search name

Bovine Fibronectin ELISA Kit, Bovine FN ELISA Kit, Bovine fibronectin 1 ELISA Kit, Bovine CIG ELISA Kit, Bovine ED-B ELISA

Kit, Bovine FINC ELISA Kit, Bovine FNZ ELISA Kit, Bovine GFND ELISA Kit, Bovine GFND2 ELISA Kit, Bovine LETS ELISA Kit,

Bovine MSF ELISA Kit, Bovine cold-insoluble globulin ELISA Kit, Bovine migration-stimulating factor ELISA Kit

Intended use

This immunoassay kit allows for the in vitro quantitative determination of bovine fibronectin,FN concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to FN. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for FN and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain FN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of FN in the samples is then determined by comparing the O.D. of the samples to the standard curve.