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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测牛血清,血浆等样本中目标蛋白的浓度
- 适应物种:
牛
- 标记物:
Bovine Polypeptide N-acetylgalactosaminyltransferase 1,GALNT1
- 样本:
牛的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Bovine Polypeptide N- acetylgalactosaminyltransferase 1, GALNT1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
GALNT1,GALNAC-T1; GalNAc transferase 1; UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 1; UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 1 (GalNAc-T1); polypeptide GalNAc transferase 1; pp-GaNTase 1; protein-UDP acetylgalactosaminyltransferase 1; polypeptide N-acetylgalactosaminyltransferase 1
Search name
Bovine GALNT1 ELISA KIT ,Bovine GALNAC-T1 ELISA KIT ,Bovine GalNAc transferase 1 ELISA KIT ,Bovine UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 1 ELISA KIT ,Bovine UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 1 (GalNAc-T1) ELISA KIT ,Bovine polypeptide GalNAc transferase 1 ELISA KIT ,Bovine pp-GaNTase 1 ELISA KIT ,Bovine protein-UDP acetylgalactosaminyltransferase 1 ELISA KIT ,Bovine polypeptide N-acetylgalactosaminyltransferase 1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Bovine Polypeptide N- acetylgalactosaminyltransferase 1, GALNT1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Polypeptide N-acetylgalactosaminyltransferase 1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Polypeptide N-acetylgalactosaminyltransferase 1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Polypeptide N-acetylgalactosaminyltransferase 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Polypeptide N-acetylgalactosaminyltransferase 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Customized product
Delivery time 4-6month
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文献和实验The aim of this article is to present a unique in vitro folding technique for glycosyltransferases to generate active proteins that can be used for X-ray crystallographic and bioconjugation protocols. Although a number of in vitro refolding
Glycosyltransferases in Chemo-enzymatic Synthesis of Oligosaccharides
hydrophobic aglycon, 2,6-diaminopyridine (DAP), to N ,N ′-diacetylchitobiose. By the subsequent action of recombinant Caenorhabditis elegans β1,4-N -acetylgalactosaminyltransferase the substrate was efficiently converted to GalNAcβ1-4GlcNAcβ-R (LDN-R
The human and bovine 14-3-3 eta protein mRNAs are highly conservedEvolutionary conservation of the 14-3-3 proteinExpression and structural analysis of 14-3-3 proteinsCrystal structure of the zeta isoform of the 14-3-3 proteinStructure of a 14
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