Bovine Elastase ELISA Kit

Bovine Elastase ELISA Kit

Product Name:Bovine Elastase ELISA Kit
Packing:96T

Catalog No.:ELA-E1483b

Gene Name:Bovine Elastase

Detect Range:31.2-2000pg/ml

Sensitivity:18.75pg/ml

Target Protein Name:Bovine Elastase

Alternative Name:Elastase,Bovine Elastase
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Bovine Elastase ELISA Kit allows for the in vitro quantitative determination of Bovine Elastase concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Bovine Elastase ELISA Kit has been pre-coated with an Bovine Elastase antibody specific to Bovine Elastase .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Elastase and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Elastase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Elastase in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!