- ¥1580
- gelatins
- jlcR3360
- 国内
- 2025年07月16日
- WB,ELISA等
- 人/动物/植物
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 供应商：
江西江蓝纯生物试剂有限公司
- 库存：
22
- 克隆性：
单克隆
- 保质期：
1年
- 抗体英文名：
Phospho-RCC1 (Ser11)
- 抗体名：
磷酸化染色体浓缩调控蛋白1抗体
- 适应物种：
人/动物/植物
- 应用范围：
WB,ELISA等
- 浓度：
1mg/ml
- 保存条件：
-20 °
- 规格：
100ul
英文名称 : Phospho-RCC1 (Ser11)
中文名称 : 磷酸化染色体浓缩调控蛋白1抗体
别 名 : Cell cycle regulatory protein; CHC 1; CHC1; Chromosome condensation 1; Chromosome condensation protein 1; Guanine nucleotide releasing protein; HERC2; Ran GEF; RanGEF; RCC 1; RCC1 I; Regulator of chromosome condensation 1; Regulator of chromosome condensation; SHEP1; RCC1_HUMAN.
产品类型 : 磷酸化抗体
研究领域 : 细胞生物 染色质和核信号 信号转导 细胞凋亡 转录调节因子 激酶和磷酸酶
抗体来源 : Rabbit
克隆类型 : Polyclonal
交叉反应 : Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep,
产品应用 : ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 : 45kDa
细胞定位 : 细胞核 细胞浆
性 状 : Lyophilized or Liquid
浓 度 : 1mg/ml
免 疫 原 : KLH conjugated Synthesised phosphopeptide derived from human RCC1 around the phosphorylation site of Ser11:RR(p-S)PP
亚 型 : IgG
纯化方法 : affinity purified by Protein A
储 存 液 : 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 : Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed : PubMed
产品介绍 : Ran GTPase plays important roles in nucleocytoplasmic transport in interphase and in both spindle formation and nuclear envelope (NE) assembly during mitosis. The latter functions rely on the presence of high local concentrations of GTP bound Ran near mitotic chromatin. RanGTP localization has been proposed to result from the association of Ran's GDP/GTP exchange factor, RCC1, with chromatin , but Ran is shown here to bind directly to chromatin in two modes, either dependent or independent of RCC1, and, where bound, to increase the affinity of chromatin for NE membranes.
Function:
Guanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP by GTP. Involved in the regulation of onset of chromosome condensation in the S phase. Binds both to the nucleosomes and double-stranded DNA. RCC1-Ran complex (together with other proteins) acts as a component of a signal transmission pathway that detects unreplicated DNA. Plays a key role in nucleo-cytoplasmic transport, mitosis and nuclear-envelope assembly.
Subunit:
Interacts with ARRB2; the interaction is detected in the nucleus upon OR1D2 stimulation.
Subcellular Location:
Nucleus. Cytoplasm. Note=Becomes dispersed throughout the cytoplasm during mitosis.
Post-translational modifications:
N-terminal methylation by METTL11A/NTM1 is required for binding double-stranded DNA and stable chromatin association. Di-and trimethylation produce a permanent positive charge on the amino group, which facilitate electrostatic binding to the phosphate groups on DNA, while inhibiting histone-binding. Methylated tail helps retain RCC1 on chromosomes during nucleotide exchange on Ran.
Similarity:
Contains 7 RCC1 repeats.
SWISS:
P18754
Gene ID:
1104
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验饥饿的 HeLa 和 NIH 3T3 癌细胞系获得的裂解物中的磷酸化蛋白特异性。图示：从复杂生物样品中富集高纯度磷蛋白。用 theThermo Scientific Pierce 磷酸化蛋白富集试剂盒进行 Weste blot 分析，根据试剂盒说明书制备细胞裂解液，富集磷酸化蛋白。利用识别生长因子信号传导相关关键调控蛋白的磷酸化特异性抗体实现蛋白检测。细胞色素 C (pI 9.6) 和 p15Ink4b (pI 5.5) 作为非磷酸化蛋白非特异性结合的阴性对照。FT = 流穿馏分，W = 合并洗脱馏分，E
）是基于体内分析发展起来的方法，它能真实、完整地反映结合在DNA 序列上的调控蛋白，是目前确定与特定蛋白结合的基因组区域或确定与特定基因组区域结合的蛋白质的最好方法。ChiP 技术和芯片技术的结合有利于确定全基因组范围内染色体蛋白的分布模式以及组蛋白修饰情况。UPSTATE 公司是一家世界著名专门提供信号转导研究产品的生物公司，商业化ChiP 分析试剂盒是该公司的独家产品。应用： 1.组蛋白修饰酶的抗体作为" 生物标记"2.转录调控分析3.药物开发研究4.有丝分裂研究5.DNA 损失与凋亡分析
或静电作用，分子构象改变，易直线聚合成细丝，电镜下可见6条细丝进一步平行聚合成凝胶状态的螺旋链索状结构，使红细胞膜受牵张而变成镰形。 (2)朊病毒蛋白(PrP) 与朊病毒蛋白构象相关的疾病称为朊病毒病，人类有克雅氏病(CJD)、新型变种CJD(vCJD)、格斯特曼综合征(GSS)、致死性家族嗜睡症(FFI)、库鲁病(Kulu)等。不论是散发、遗传还是传染性朊病毒病都与PrP构象转换有关，即由生理性PrPc转变为病理性PrPsc[2]。PrPsc是正常细胞表面蛋白质(PrPc)的构象异构体，两者之间
