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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Atg12
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Atg12 ELISA Kit
Catalog EF014267
Packing 48Tests/96Tests
ELISA Method Sandwich ELISA Species Mouse
Sample Mouse serum, plasma, tissue homogenates and other biological fluids and Recombinant Atg12
Detect Range 0.156-10ng/ml sensitivity 0.094ng/ml
ELISA Intend Use
Mouse Atg12 ELISA Kit is a Sandwich ELISA KIT detecting the concentration of Ubiquitin-like protein ATG12 in Mouse serum, plasma, tissue homogenates and other biological fluids.
(ELISA KIT is only for Research Only, not for the diagnostic Use!)
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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
)9 Incubate 30 min at 37°C.10 Rinse 3x with 0.05% Tween 80 in 0.01 M PBS, pH 7.4.11 Rinse 3x with HPLC water.12 Add 50 µl of color development reagent(1 tablet of p-nitrophenyl reagent in 5 ml of 10% diethanolamine, pH 9.8) (mix well)13 Incubate 30 min at 37°C
results. Incubate. 3. Dump out the antigen solution and add 100 µL of blocking buffer (1% BSA, 0.1 M KPi, 0.1% Tween-20, 0.02% thimerisol, pH 7). If your carrier protein for injection was BSA, then substitute 1% non-fat dry milk for the BSA. Incubate
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