26S proteasome complex subunit
DSS1, Shfm1 ELISA KIT/ Mouse 26S proteasome complex subunit DSS1, Shfm1 ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-08672m
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白26S proteasome complex subunit DSS1, Shfm1含量
- 适应物种：
小鼠
- 标记物：
26S proteasome complex subunit DSS1, Shfm1
- 样本：
小鼠血清，血浆，组织匀浆及其他生物样本
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse 26S proteasome complex subunit DSS1, Shfm1 ELISA KIT
Product Name:Mouse 26S proteasome complex subunit DSS1, Shfm1 ELISA KIT
Packing:96T
Catalog No.:ELI-08672m
Gene Name:Mouse Shfm1
Detect Range:78-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse Shfm1
Alternative Name:Mouse 26S proteasome complex subunit DSS1, Shfm1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse 26S proteasome complex subunit DSS1, Shfm1 ELISA KIT allows for the in vitro quantitative determination of Mouse Shfm1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse 26S proteasome complex subunit DSS1, Shfm1 ELISA KIT has been pre-coated with an Mouse 26S proteasome complex subunit DSS1, Shfm1 antibody specific to Mouse Shfm1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Shfm1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Shfm1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Shfm1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Assays for Proteasome Inhibition
concentration in all mammalian cells. The ATP hydrolytic activity and the specific subunits that bind ubiquitin in the 26S are located within a protein complex known as the 19S subunit which caps either end of the 20S core. The ATP-independent proteolytic
Studies of the Ubiquitin Proteasome System
of Small Interfering RNAs (siRNAs) to Reduce Expression of Ubiquitin‐Proteasome System Proteins in Cells Basic Protocol 13: Localizing Degradation to the Proteasome The NEDD8 Conjugation System Basic Protocol 14: Purification of APP‐BP1/Uba3 Complex (E1
Assays for Proteasome Assembly and Maturation
The 20S proteasome is a complex multisubunit protease that is present in all phylae of life. Eukaryotic 26S proteasomes, which are composed of 20S proteasomes and 19S activator complexes, mediate the degradation of ubiquitylated proteins
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