Toll-like receptor 11, Tlr11 E

Mouse Toll- like receptor 11, Tlr11 ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Tlr11,Gm287; toll-like receptor

Search name

Mouse Tlr11 ELISA KIT ,Mouse Gm287 ELISA KIT ,Mouse toll-like receptor ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Mouse Toll- like receptor 11, Tlr11 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tlr11. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Tlr11 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Tlr11, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Tlr11 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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