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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Transmembrane protein 52, Tmem52
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Transmembrane protein 52, Tmem52 ELISA KIT
Product Name:Mouse Transmembrane protein 52, Tmem52 ELISA KIT
Packing:96T
Catalog No.:ELI-51656m
Gene Name:Mouse Tmem52
Detect Range:15.6-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Mouse Tmem52
Alternative Name:Mouse Transmembrane protein 52, Tmem52
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Transmembrane protein 52, Tmem52 ELISA KIT allows for the in vitro quantitative determination of Mouse Tmem52 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Transmembrane protein 52, Tmem52 ELISA KIT has been pre-coated with an Mouse Transmembrane protein 52, Tmem52 antibody specific to Mouse Tmem52 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Tmem52 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Tmem52 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Tmem52 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
Purification Kit according to the manufacturer’s instructions. Alternatively, standard plasmid preparation protocols or kits can also be used. Screening for Ligated Partners 53. Screen the purified plasmids (from Step 52
Semiquantitative immunoblots of membrane protein-epidermal growth factor
Membrane proteins or cytokines are sometimes difficult to isolate and purify. Our group recently concentrated on epidermal growth factor (EGF) protein expression studies. Mature EGF was initially identified from mouse submaxillary gland extract
Methods to Detect Protein Glutathionylation
(PBS; appendix 2A ), ice cold Cell lysis buffer (see recipe ), ice cold Bicinchoninic acid (BCA) protein assay kit
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