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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Ubiquitin-like protein 4A, Ubl4a
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Ubiquitin- like protein 4A, Ubl4a ELISA KIT
Product Name:Mouse Ubiquitin- like protein 4A, Ubl4a ELISA KIT
Packing:96T
Catalog No.:ELI-51310m
Gene Name:Mouse Ubl4a
Detect Range:15.6-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Mouse Ubl4a
Alternative Name:Mouse Ubiquitin-like protein 4A, Ubl4a
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Ubiquitin- like protein 4A, Ubl4a ELISA KIT allows for the in vitro quantitative determination of Mouse Ubl4a concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Ubiquitin- like protein 4A, Ubl4a ELISA KIT has been pre-coated with an Mouse Ubiquitin-like protein 4A, Ubl4a antibody specific to Mouse Ubl4a .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Ubl4a and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Ubl4a , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Ubl4a in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Studies of the Ubiquitin Proteasome System
studied outcome of ubiquitylation, we now understand that modification of proteins with ubiquitin has numerous other cellular roles that alter protein function and that are unrelated to proteasomal degradation. Ubiquitylation is a complex process
Membrane‐Based Yeast Two‐Hybrid System to Detect Protein Interactions
2H) system presented in this unit overcomes some of these limitations. It is based on the split?ubiquitin protein complementation assay and detects protein interactions directly at the membrane, thereby allowing the use of full?length integral
Overview of Protein Microarrays
samples. Anal. Biochem. 414:99‐102. Guo, A. and Zhu, X.‐Y. 2006. The critical role of surface chemistry in protein microarrays. In Functional Protein Microarrays in Drug
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