Cytochrome P450 1B1, Cyp1b1 EL

Mouse Cytochrome P450 1B1, Cyp1b1 ELISA KIT

96 Tests

Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

1.14.14.1,Cytochrome P450 1B1,CYPIB1,Cytochrome P450RAP, CYP1B1,CP1B; CYPIB1; GLC3A; P4501B1; aryl hydrocarbon hydroxylase; cytochrome P450, subfamily I (dioxin-inducible), polypeptide 1 (glaucoma 3, primary infantile); flavoprotein-linked monooxygenase; microsomal monooxygenase; xenobiotic monooxygenase; cytochrome P450, family 1, subfamily B, polypeptide 1

Search name

Mouse Class 1.14.14.1 ELISA KIT ,Mouse Cytochrome P450 1B1 ELISA KIT ,Mouse CYPIB1 ELISA KIT ,Mouse Cytochrome P450RAP ELISA KIT ,Mouse  CYP1B1 ELISA KIT ,Mouse CP1B ELISA KIT ,Mouse CYPIB1 ELISA KIT ,Mouse GLC3A ELISA KIT ,Mouse P4501B1 ELISA KIT ,Mouse aryl hydrocarbon hydroxylase ELISA KIT ,Mouse cytochrome P450 ELISA KIT ,Mouse  subfamily I dioxin-inducible ELISA KIT ,Mouse  polypeptide 1 glaucoma 3 primary infantile ELISA KIT ,Mouse flavoprotein-linked monooxygenase ELISA KIT ,Mouse microsomal monooxygenase ELISA KIT ,Mouse xenobiotic monooxygenase ELISA KIT ,Mouse cytochrome P450 family 1 subfamily B polypeptide 1 ELISA KIT ,

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Mouse Cytochrome P450 1B1 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle

The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 1B1, During the reaction, Cytochrome P450 1B1 in the sample or standard competes with a fixed amount of biotin-labeled Cytochrome P450 1B1 for sites on a pre-coated Monoclonal antibody specific to Cytochrome P450 1B1. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Cytochrome P450 1B1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.