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- 详细信息
- 文献和实验
- 技术资料
- 库存:
10
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Pyruvate dehydrogenase E1 component subunit alpha, testis-
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Pyruvate dehydrogenase E1 component subunit alpha, testis- specific form, mitochondrial, PDHA2 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial,; PDHE1-A type II,PDHA2,PDHAL; PDHE1-A type II; pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial; pyruvate dehydrogenase, E1-alpha polypeptide, testis specific; pyruvate dehydrogenase (lipoamide) alpha 2
Search name
Mouse Pyruvate dehydrogenase E1 component subunit alpha ELISA KIT ,Mouse testis-specific form ELISA KIT ,Mouse mitochondrial ELISA KIT ,Mouse ELISA KIT ,Mouse PDHE1-A type II ELISA KIT ,Mouse PDHA2 ELISA KIT ,Mouse PDHAL ELISA KIT ,Mouse PDHE1-A type II ELISA KIT ,Mouse pyruvate dehydrogenase E1 component subunit alpha ELISA KIT ,Mouse testis-specific form ELISA KIT ,Mouse mitochondrial ELISA KIT ,Mouse pyruvate dehydrogenase ELISA KIT ,Mouse E1-alpha polypeptide ELISA KIT ,Mouse testis specific ELISA KIT ,Mouse pyruvate dehydrogenase (lipoamide) alpha 2 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse pyruvate dehydrogenase e1 component subunit alpha, testis-specific form, mitochondrial,PDHE1-A type II concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to PDHE1-A type II. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for PDHE1-A type II and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain PDHE1-A type II, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PDHE1-A type II in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
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