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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Cysteine and glycine-rich protein 1, Csrp1
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Cysteine and glycine- rich protein 1, Csrp1 ELISA KIT
Product Name:Mouse Cysteine and glycine- rich protein 1, Csrp1 ELISA KIT
Packing:96T
Catalog No.:ELI-50615m
Gene Name:Mouse Csrp1
Detect Range:0.15-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse Csrp1
Alternative Name:Mouse Cysteine and glycine-rich protein 1, Csrp1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Cysteine and glycine- rich protein 1, Csrp1 ELISA KIT allows for the in vitro quantitative determination of Mouse Csrp1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Cysteine and glycine- rich protein 1, Csrp1 ELISA KIT has been pre-coated with an Mouse Cysteine and glycine-rich protein 1, Csrp1 antibody specific to Mouse Csrp1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Csrp1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Csrp1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Csrp1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验----------------------- Screening for Protein-Protein Interactions. Protein G was from Pierce (Rockford, IL) and BODIPY-FL-Goat-anti-Mouse IgG was from Molecular Probes (Eugene, OR). IKBalpha and p50 were kindly provided by T. Maniatis (Harvard University, Cambridge, MA
Studies of the Ubiquitin Proteasome System
Basic Protocol 9: Substrate Ubiquitylation by Multisubunit E3s Materials VBC‐Cul2 E3 protein complex expressed and purified using baculoviral expression system ( protocol 45 ) Mouse E1
Analysis of Oxidative Modification of Proteins
has thus often been employed for the quantification of generalized protein oxidation. Besides carbonylation, other types of oxidative damage that have been investigated in depth are the modifications of cysteine, tyrosine, and aspartate, or asparagine residues
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