Rho-related GTP-binding protei
n RhoN, Rnd2 ELISA KIT/ Mouse Rho-related GTP-binding protein RhoN, Rnd2 ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-42655m
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Rho-related GTP-binding protein RhoN, Rnd2
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Rho- related GTP- binding protein RhoN, Rnd2 ELISA KIT
Product Name:Mouse Rho- related GTP- binding protein RhoN, Rnd2 ELISA KIT
Packing:96T
Catalog No.:ELI-42655m
Gene Name:Mouse Rnd2
Detect Range:78.1-5000pg/ml
Sensitivity:46.875pg/ml
Target Protein Name:Mouse Rnd2
Alternative Name:Mouse Rho-related GTP-binding protein RhoN, Rnd2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Rho- related GTP- binding protein RhoN, Rnd2 ELISA KIT allows for the in vitro quantitative determination of Mouse Rnd2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Rho- related GTP- binding protein RhoN, Rnd2 ELISA KIT has been pre-coated with an Mouse Rho-related GTP-binding protein RhoN, Rnd2 antibody specific to Mouse Rnd2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Rnd2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Rnd2 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Rnd2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验小GTP结合蛋白(small GTP binding protein)
), 它催化GDT同GTP的交换。另一种是GTP 酶激活蛋白(GTPase-activating protein, GAP), 它触发结合的GTP水解。 许多小GTP结合蛋白都有一个共价结合的脂基团, 帮助它们同膜结合。小GTP蛋白包括ras、rab和rho蛋白, 它们在一些重要的的活动中起调节作用, 包括分泌活动和细胞骨架的装配等。
Activation of Heterotrimeric GTP-Binding Proteins Upon TCR/CD3 Engagement
that the signaling pathways involve protein tyrosine kinases and phosphatases (4 ), the Rho family of small guanosine triphosphate (GTP)-binding proteins (5 ), and the heterotrimeric GTP-binding proteins (G proteins) of the Gq family (6 ,7 ). The involvement of G
Biochemical Assays to Characterize Rho GTPases
of binding on the protein structure and, ultimately, to gain insights into their biological functions. This chapter describes (1) detailed protocols for the expression and purification of Rho GTPases, of �effector-binding domains, and catalytic domains
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