ATP synthase subunit b, mitoch

Mouse ATP synthase subunit b, mitochondrial, Atp5f1 ELISA KIT

Product Name:Mouse ATP synthase subunit b, mitochondrial, Atp5f1 ELISA KIT
Packing:96T

Catalog No.:ELI-49593m

Gene Name:Mouse Atp5f1

Detect Range:31.25-2000pg/ml

Sensitivity:18.75pg/ml

Target Protein Name:Mouse Atp5f1

Alternative Name:Mouse ATP synthase subunit b, mitochondrial, Atp5f1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Mouse ATP synthase subunit b, mitochondrial, Atp5f1 ELISA KIT allows for the in vitro quantitative determination of Mouse Atp5f1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Mouse ATP synthase subunit b, mitochondrial, Atp5f1 ELISA KIT has been pre-coated with an Mouse ATP synthase subunit b, mitochondrial, Atp5f1 antibody specific to Mouse Atp5f1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Atp5f1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Atp5f1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Atp5f1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!