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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Anterior gradient protein 3 homolog, Agr3
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Anterior gradient protein 3 homolog, Agr3 ELISA KIT
Product Name:Mouse Anterior gradient protein 3 homolog, Agr3 ELISA KIT
Packing:96T
Catalog No.:ELI-49543m
Gene Name:Mouse Agr3
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Agr3
Alternative Name:Mouse Anterior gradient protein 3 homolog, Agr3
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Anterior gradient protein 3 homolog, Agr3 ELISA KIT allows for the in vitro quantitative determination of Mouse Agr3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Anterior gradient protein 3 homolog, Agr3 ELISA KIT has been pre-coated with an Mouse Anterior gradient protein 3 homolog, Agr3 antibody specific to Mouse Agr3 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Agr3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Agr3 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Agr3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验(one vial) dissolve in 14.7 ml sterile saline (0.9% NaCl) filter to sterilize if desired aliquot into eppendorf tubes and store at -20 degrees Centrigrade 2. Inject into the Tibialis anterior muscle of >20 g mouse as follows: 100 µl of 10 µM
specificity of each fusion protein was tested by ELISA using a panel of DNA substrates (Fig. 2B,C). The predicted DNA binding site of each TFZF was decoded from the -helical sequence of the corresponding zinc finger (Table 1). As expected, the majority
v468.chapter 1 经典 Wnt/b-Catenin 信号通路 (英文完整版)
., Wagenaar, E.,Parren, P., Weigel, D., and Nusse R. (1987) The Drosophila homolog of the mouse mammary oncogene int-1 is identical to the segment polarity gene wingless. Cell 50 , 649–657. 3. McMahon, A. P. and Moon, R. T. (1989) Ectopic expression
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