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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Homeobox protein Hox-A3, Hoxa3
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Homeobox protein Hox- A3, Hoxa3 ELISA KIT
Product Name:Mouse Homeobox protein Hox- A3, Hoxa3 ELISA KIT
Packing:96T
Catalog No.:ELI-48277m
Gene Name:Mouse Hoxa3
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Hoxa3
Alternative Name:Mouse Homeobox protein Hox-A3, Hoxa3
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Homeobox protein Hox- A3, Hoxa3 ELISA KIT allows for the in vitro quantitative determination of Mouse Hoxa3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Homeobox protein Hox- A3, Hoxa3 ELISA KIT has been pre-coated with an Mouse Homeobox protein Hox-A3, Hoxa3 antibody specific to Mouse Hoxa3 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Hoxa3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Hoxa3 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Hoxa3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Membrane‐Based Yeast Two‐Hybrid System to Detect Protein Interactions
A3 amino acids of S. cerevisiae STE2 protein; Ost4, entire open reading frame of S. cerevisiae Ost4 protein; MCS, multiple cloning site. (E ) Multiple cloning sites for MbY2H vectors. View Image
体 的两个拷贝,分别来自父母本。 Homomultimeric protein (同源多聚体蛋白质):由相同亚基组成的蛋白质。 Homozygote (纯合体):同源染色体相应位点有相同等位基因的个体。 Hotspot (热点):突变或者重组频率显著增加的位点。 Housekeeping (constitutive) genes (持家或组成型基因):是那些(理论上)在所有细胞中都表 达的基因,因为其功能对任何细胞型都是必要的。 HOX genes (HOX 基因):包括
Enzyme-Linked ImmunoSorbent Assay (ELISA) for NGF
A. DAY 1 PROCEDURE• ABSORPTION OF THE POLYCLONAL AND PREIMMUNE SERUMThe Goat Anti-Mouse (GAM) NGF polyclonal antibody recognizes 2.5B NGF and the Goat IgG (GIG) preimmune serum serves as a control for the antibody (Hazleton). The relative signal
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