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- 详细信息
- 文献和实验
- 技术资料
- 库存:
10
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Eukaryotic translation initiation factor 1, Eif1
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Eukaryotic translation initiation factor 1, Eif1 ELISA KIT
Product Name:Mouse Eukaryotic translation initiation factor 1, Eif1 ELISA KIT
Packing:96T
Catalog No.:ELI-47582m
Gene Name:Mouse Eif1
Detect Range:78.1-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse Eif1
Alternative Name:Mouse Eukaryotic translation initiation factor 1, Eif1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Eukaryotic translation initiation factor 1, Eif1 ELISA KIT allows for the in vitro quantitative determination of Mouse Eif1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Eukaryotic translation initiation factor 1, Eif1 ELISA KIT has been pre-coated with an Mouse Eukaryotic translation initiation factor 1, Eif1 antibody specific to Mouse Eif1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Eif1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Eif1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Eif1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Genome‐Wide Annotation and Quantitation of Translation by Ribosome Profiling
reading frames, an adaptation that reveals the sites of translation initiation by pre?treating cells with harringtonine to immobilize initiating ribosomes is described. The protocol described requires 5 to 7 days to generate a completed ribosome profiling
[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
protein), beta 1, 88kDa (CTNNB1), mRNA 1165 NM_003753 Homo sapiens eukaryotic translation initiation factor 3, subunit 7 zeta, 66/67kDa (EIF3S7), mRNA 1363 NM_004541 Homo sapiens NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 1, 7.5kDa (NDUFA1
, a mammalian member of the RDE-1 family has been identified as a translation initiation factor. Interestingly, Arabidopsis mutants of AGO1, which are defective for cosuppression, also exhibit defects in leaf development. Thus some processes or enzymes involved
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