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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Protein FAM13B, Fam13b
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Protein FAM13B, Fam13b ELISA KIT
Product Name:Mouse Protein FAM13B, Fam13b ELISA KIT
Packing:96T
Catalog No.:ELI-32785m
Gene Name:Mouse Fam13b
Detect Range:31.25-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Fam13b
Alternative Name:Mouse Protein FAM13B, Fam13b
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Protein FAM13B, Fam13b ELISA KIT allows for the in vitro quantitative determination of Mouse Fam13b concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Protein FAM13B, Fam13b ELISA KIT has been pre-coated with an Mouse Protein FAM13B, Fam13b antibody specific to Mouse Fam13b .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Fam13b and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Fam13b , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Fam13b in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人B 淋巴细胞刺激因子 (BAFF)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 BAFF( BAFF/BLyS/TNFSF13B ) 单抗包被于酶标板上,标准品和样品中的 BAFF
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 小鼠B 淋巴细胞刺激因子 (BAFF)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗小鼠 BAFF( BAFF/BLyS/TNFSF13B ) 单抗包被于酶标板上,标准品和样品
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