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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Diacylglycerol O-acyltransferase 2, Dgat2
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Diacylglycerol O- acyltransferase 2, Dgat2 ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
DGAT2,ARAT; GS1999FULL; acyl-CoA retinol O-fatty-acyltransferase; diacylglycerol O-acyltransferase homolog 2; diacylglycerol O-acyltransferase-like protein 2; diglyceride acyltransferase 2; retinol O-fatty-acyltransferase; diacylglycerol O-acyltransferase 2
Search name
Mouse DGAT2 ELISA KIT ,Mouse ARAT ELISA KIT ,Mouse GS1999FULL ELISA KIT ,Mouse acyl-CoA retinol O-fatty-acyltransferase ELISA KIT ,Mouse diacylglycerol O-acyltransferase homolog 2 ELISA KIT ,Mouse diacylglycerol O-acyltransferase-like protein 2 ELISA KIT ,Mouse diglyceride acyltransferase 2 ELISA KIT ,Mouse retinol O-fatty-acyltransferase ELISA KIT ,Mouse diacylglycerol O-acyltransferase 2 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse Diacylglycerol O- acyltransferase 2, Dgat2 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dgat2, During the reaction, Dgat2 in the sample or standard competes with a fixed amount of biotin-labeled Dgat2 for sites on a pre-coated Monoclonal antibody specific to Dgat2. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Dgat2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Customized Product
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文献和实验Performing Bronchoalveolar Lavage in the Mouse
and inflammatory cell populations; it is indispensable for studying cell influx in disease models of the airways such as asthma and COPD. Cell counts can be combined with methods such as ELISA, immunoblot, immunohistochemistry, quantitative polymerase chain
Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
isolation kit, Qiagen), pooled mRNA for each treatment group. As well as GAPDH, factor VII, glucose-6-phosphatase and VEGF mRNAs were also used for normalization. ELISA was used to quantify the reduction of apoB-100 protein levels in mouse plasma
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