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- 详细信息
- 文献和实验
- 技术资料
- 灵敏度:
0.078ng/mL
- 库存:
50
- 供应商:
LSM Bio
- 检测范围:
0.156-10 ng/mL
- 检测方法:
夹心法ELISA
- 应用:
检测 小鼠样本Mouse - binding inhibitor- like 5, Dbil55蛋白浓度
- 适应物种:
小鼠
- 标记物:
Mouse - binding inhibitor- like 5, Dbil5
- 样本:
小鼠血清,血浆,组织匀浆
- 规格:
96T
Mouse - binding inhibitor- like 5, Dbil5 ELISA KIT
Product Name:Mouse - binding inhibitor- like 5, Dbil5 ELISA KIT
Packing:96T
Catalog No.:ELI-46695m
Gene Name:Mouse Dbil5
Detect Range:0.156-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse Dbil5
Alternative Name:Mouse -binding inhibitor-like 5, Dbil5
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse - binding inhibitor- like 5, Dbil5 ELISA KIT allows for the in vitro quantitative determination of Mouse Dbil5 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse - binding inhibitor- like 5, Dbil5 ELISA KIT has been pre-coated with an Mouse -binding inhibitor-like 5, Dbil5 antibody specific to Mouse Dbil5 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Dbil5 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Dbil5 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Dbil5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Upregulation of Antioxidant Capacity and Nucleotide Precursor Availability Suffices for Oncogenic Transformation
Author:YangZhang1YiXu1WenyunLu23Jonathan M.Ghergurovich24LiliGuo56Ian A.Blair5Joshua D.Rabinowitz23XiaoluYang17
Received 22 January 2020, Revised 4 August 2020, Accepted 30 September 2020, Available online 6 November 2020.
Published: November 6, 2020
Cell Metabolism
Available online 6 November 2020 In Press
doi: doi.org/10.1016/j.cmet.2020.10.002
RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
Capture ELISA Quantitation of Mouse Adenovirus Type 1 in Infected Organs
A capture enzyme-linked immunosorbent assay (ELISA) was optimized for identification of mouse adenovirus type 1 in infected brain homogenates. The ELISA method allows for a much faster quantitation of virus in infected organs than plaque
, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
技术资料



