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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Ig gamma-2A chain C region, A allele, Ighg
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Ig gamma- 2A chain C region, A allele, Ighg ELISA KIT
Product Name:Mouse Ig gamma- 2A chain C region, A allele, Ighg ELISA KIT
Packing:96T
Catalog No.:ELI-47186m
Gene Name:Mouse Ighg
Detect Range:78.1-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse Ighg
Alternative Name:Mouse Ig gamma-2A chain C region, A allele, Ighg
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Ig gamma- 2A chain C region, A allele, Ighg ELISA KIT allows for the in vitro quantitative determination of Mouse Ighg concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Ig gamma- 2A chain C region, A allele, Ighg ELISA KIT has been pre-coated with an Mouse Ig gamma-2A chain C region, A allele, Ighg antibody specific to Mouse Ighg .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Ighg and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Ighg , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Ighg in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验, dry 5. block half of the plate (with platelets) by PBS-BSA 1% and coat another half by glycine (x1) - BSA for 1 hour at 37ѓC 6. wash 3 times in PBS-tween 20, dry 7. incubated at 37ѓc for 1 hour with a 1:1000 dilution of rat IgG2a anti-mouse kappa
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: Total mouse IgG Ig Type: mouse IgG specific goat IgG Applications: Western blot ELISA Preparation Produced in goats immunized with purified total mouse IgG. Mouse IgG1, mouse IgG2A, mouse IgG2B and mouse IgG3 specific IgG was purified by mouse IgG
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