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- 详细信息
- 文献和实验
- 技术资料
- 库存:
10
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Cytochrome c oxidase subunit 3, Mtco3
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Cytochrome c oxidase subunit 3, Mtco3 ELISA KIT
Product Name:Mouse Cytochrome c oxidase subunit 3, Mtco3 ELISA KIT
Packing:96T
Catalog No.:ELI-47165m
Gene Name:Mouse Mtco3
Detect Range:15.6-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Mouse Mtco3
Alternative Name:Mouse Cytochrome c oxidase subunit 3, Mtco3
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Cytochrome c oxidase subunit 3, Mtco3 ELISA KIT allows for the in vitro quantitative determination of Mouse Mtco3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Cytochrome c oxidase subunit 3, Mtco3 ELISA KIT has been pre-coated with an Mouse Cytochrome c oxidase subunit 3, Mtco3 antibody specific to Mouse Mtco3 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Mtco3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Mtco3 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Mtco3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验线粒体荧光探针大全:TMRM,Mitotracker,JC-1
to cytochrome oxidase (Complex IV), the relative levels of all OxPhos enzyme complexes in normal and diseased tissues can be evaluated. The mouse monoclonal 7H10 anti�OxPhos Complex V subunit (bovine) (anti�F1 F0 -ATPase subunit , A21350) and mouse monoclonal
[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
, H+ transporting, lysosomal interacting protein 1 (ATP6IP1), mRNA 1089 NM_003526 Homo sapiens H2B histone family, member L (H2BFL), mRNA 2232 NM_004718 Homo sapiens cytochrome c oxidase subunit VIIa polypeptide 2 like (COX7A2L), nuclear gene
级联反应:细胞色素C/Apaf-1复合物激活caspase-9,后者再激活caspase-3和其它下游caspase。细胞色素C氧化酶亚单位Ⅳ(cytochrome c oxidase subunit Ⅳ:COX4)是定位在线粒体内膜上的膜蛋白,凋亡发生时,它保留在线粒体内,因而它是线粒体富集部分的一个非常有用的标志。 ApoAlertTMCell Fractionation Kit不用超离心,可从凋亡和非凋亡细胞中快速有效分离出高度富集的线粒体部分,再进一步通过Western杂交
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