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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Emopamil-binding protein-like, Ebpl
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Emopamil- binding protein- like, Ebpl ELISA KIT
Product Name:Mouse Emopamil- binding protein- like, Ebpl ELISA KIT
Packing:96T
Catalog No.:ELI-47153m
Gene Name:Mouse Ebpl
Detect Range:31.25-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Ebpl
Alternative Name:Mouse Emopamil-binding protein-like, Ebpl
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Emopamil- binding protein- like, Ebpl ELISA KIT allows for the in vitro quantitative determination of Mouse Ebpl concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Emopamil- binding protein- like, Ebpl ELISA KIT has been pre-coated with an Mouse Emopamil-binding protein-like, Ebpl antibody specific to Mouse Ebpl .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Ebpl and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Ebpl , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Ebpl in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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[3 H]flumazenil binding to mouse brain membranes. (A ) Analysis of [3 H]flumazenil saturation binding. Membranes prepared from whole mouse brains (∼0.1 mg protein/tube) were incubated in 50 mM Tris‐citrate, pH 7.4, containing various concentrations
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