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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Transducin-like enhancer protein 6, Tle6
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Transducin- like enhancer protein 6, Tle6 ELISA KIT
Product Name:Mouse Transducin- like enhancer protein 6, Tle6 ELISA KIT
Packing:96T
Catalog No.:ELI-45901m
Gene Name:Mouse Tle6
Detect Range:78.1-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse Tle6
Alternative Name:Mouse Transducin-like enhancer protein 6, Tle6
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Transducin- like enhancer protein 6, Tle6 ELISA KIT allows for the in vitro quantitative determination of Mouse Tle6 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Transducin- like enhancer protein 6, Tle6 ELISA KIT has been pre-coated with an Mouse Transducin-like enhancer protein 6, Tle6 antibody specific to Mouse Tle6 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Tle6 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Tle6 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Tle6 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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: 3128136151
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文献和实验Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
Identification of Protein Interactions by Far Western Analysis
protein of interest cloned into an in vitro expression vector In vitro transcription/translation kit (Promega) 10 mCi/ml 35
-transducin repeat-containing protein (ß-TrCP), human (10) GUGGAAUUUGUGGAACAUCTT 293 TSG101 (a ubiquitin enzyme 2 variant protein) (14) 413-433 AACCUCCAGUCUUCUCUCGUC Human HeLa-M & HeLa KK3 VASP (1) XM_009141 1087-1109 aaagaggaaatcattgaagcc Human HeLa
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