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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Ribonuclease-like protein 9, Rnase9
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Ribonuclease- like protein 9, Rnase9 ELISA KIT
Product Name:Mouse Ribonuclease- like protein 9, Rnase9 ELISA KIT
Packing:96T
Catalog No.:ELI-44967m
Gene Name:Mouse Rnase9
Detect Range:78.1-5000pg/ml
Sensitivity:46.875pg/ml
Target Protein Name:Mouse Rnase9
Alternative Name:Mouse Ribonuclease-like protein 9, Rnase9
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Ribonuclease- like protein 9, Rnase9 ELISA KIT allows for the in vitro quantitative determination of Mouse Rnase9 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Ribonuclease- like protein 9, Rnase9 ELISA KIT has been pre-coated with an Mouse Ribonuclease-like protein 9, Rnase9 antibody specific to Mouse Rnase9 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Rnase9 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Rnase9 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Rnase9 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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: 3128136151
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文献和实验Use of Internaland External Standardsor Reference RNAs for A
, respectively, in a MAXIscript in vitro transcription reaction. 4 x 104 cpm of each probe was added to 5 µg total RNA from different mouse tissues and processed in a multiple probe ribonuclease protection assay using the RPA II Kit. Results show a comparison
Use of Internal and External Standards or Reference RNAs for
a Ribonuclease Protection Assay. High specific activity antisense RNA probes (1.15 x 10 9 cpm/µg) to GAPDH, ß-actin and cyclophilin were synthesized from Ambion's pTRI-GAPDH, pTRI-beta-actin, and pTRI-cyclophilin mouse DNA
Comparative Protein Structure Modeling Using MODELLER
Figure 2.9.12 Typical errors in comparative modeling. (A ) Errors in side chain packing. The Trp 109 residue in the crystal structure of mouse cellular retinoic acid binding protein I (red) is compared
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