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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Voltage-dependent anion-selective channel protein 1, Vda
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Voltage- dependent anion- selective channel protein 1, VDAC1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
VDAC1,VDAC,PORIN; VDAC-1; outer mitochondrial membrane protein porin 1; plasmalemmal porin; porin 31HL; porin 31HM; voltage-dependent anion-selective channel protein 1; voltage-dependent anion channel 1
Search name
Mouse VDAC1 ELISA KIT ,Mouse VDAC ELISA KIT ,Mouse PORIN ELISA KIT ,Mouse VDAC-1 ELISA KIT ,Mouse outer mitochondrial membrane protein porin 1 ELISA KIT ,Mouse plasmalemmal porin ELISA KIT ,Mouse porin 31HL ELISA KIT ,Mouse porin 31HM ELISA KIT ,Mouse voltage-dependent anion-selective channel protein 1 ELISA KIT ,Mouse voltage-dependent anion channel 1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse Voltage- dependent anion- selective channel protein 1, VDAC1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to VDAC1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for VDAC1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain VDAC1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of VDAC1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Customized product
:
:
: 3128136151
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文献和实验Photoswitchable Voltage-Gated Ion Channels
to the protein of interest by connecting the photoisomerizable moiety to a specific agonist or antagonist. Two classes of azobenzene-containing photoswitches have been developed for exogenously expressed or endogenous voltage-gated K+ channels. In both cases
Tertiary and Quaternary Structure Formation of Voltage-Gated Potassium Channels
into a cysteine-free voltage-gated potassium channel protein, using bifunctional crosslinking agents, and using an assay of the crosslinked products to determine folding/assembly events. A biogenic intermediate (i.e., nascent chain attached to transfer RNA
The ability to record the real-time movement of a protein is quite enthralling; in effect, this is what we achieve when we record the activity of an individual ion channel. Clearly we do not “see” the amino acids that make
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