- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-43443m
- 2026年03月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
10
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Ubiquitin-like protein ISG15, Isg15
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Ubiquitin- like protein ISG15, ISG15 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Ubiquitin-like protein ISG15,; Ubiquitin cross-reactive protein,Interferon-induced 15 kDa protein,Interferon-induced 17 kDa protein, ISG15,ISG15 ubiquitin-like modifier; G1P2; IFI15; IP17; UCRP; hUCRP; interferon alpha-inducible protein clone IFI-15K;
interferon-stimulated protein 15 kDa;
Search name
Mouse Ubiquitin-like protein ISG15 ELISA KIT , Mouse Ubiquitin cross-reactive protein ELISA KIT ,Mouse Interferon-induced 15 kDa protein ELISA KIT ,Mouse Interferon-induced 17 kDa protein ELISA KIT ,Mouse ISG15 ELISA KIT ,Mouse ISG15 ubiquitin-like modifier ELISA KIT ,Mouse G1P2 ELISA KIT ,Mouse IFI15 ELISA KIT ,Mouse IP17 ELISA KIT ,Mouse UCRP ELISA KIT ,Mouse hUCRP ELISA KIT ,Mouse interferon alpha-inducible protein clone IFI-15K ELISA KIT ,Mouse interferon-stimulated protein 15 kDa ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse Ubiquitin-like protein ISG15 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ubiquitin-like protein ISG15. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Ubiquitin-like protein ISG15 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Ubiquitin-like protein ISG15, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Ubiquitin-like protein ISG15 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Studies of the Ubiquitin Proteasome System
studied outcome of ubiquitylation, we now understand that modification of proteins with ubiquitin has numerous other cellular roles that alter protein function and that are unrelated to proteasomal degradation. Ubiquitylation is a complex process
Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
Membrane‐Based Yeast Two‐Hybrid System to Detect Protein Interactions
2H) system presented in this unit overcomes some of these limitations. It is based on the split?ubiquitin protein complementation assay and detects protein interactions directly at the membrane, thereby allowing the use of full?length integral
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