- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-42093m
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Mettl3
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Mettl3 ELISA KIT
Product Name:Mouse Mettl3 ELISA KIT
Packing:96T
Catalog No.:ELI-42093m
Gene Name:Mouse Mettl3
Detect Range:31.25-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Mettl3
Alternative Name:Mouse Mettl3
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Mettl3 ELISA KIT allows for the in vitro quantitative determination of Mouse Mettl3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Mettl3 ELISA KIT has been pre-coated with an Mouse Mettl3 antibody specific to Mouse Mettl3 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Mettl3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Mettl3 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Mettl3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
Nat Commun：肖东/孙妍课题组合作揭示 m6A 修饰调节细胞自噬的新机制
的 RNA 修饰可响应不断变化的营养信号实现迅速的基因表达调控，对自噬发挥重要影响，并且一些表观转录组成员在此过程中可能发挥关键作用。 该研究首先基于原代分离的小鼠胚胎成纤维细胞 (mouse embryonic fibroblasts, MEFs)，通过蛋白组学检测，发现在营养缺乏期间，m6A 阅读器 YTHDF3 表达显著上调（图 1a）。进一步的分子和细胞功能实验表明，YTHDF3 对自噬诱导至关重要，YTHDF3 过表达促进营养缺乏时自噬体形成和溶酶体降解（图 1c-l）。 图
, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
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