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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Small proline-rich protein 3, Sprr3
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Small proline- rich protein 3, Sprr3 ELISA KIT
Product Name:Mouse Small proline- rich protein 3, Sprr3 ELISA KIT
Packing:96T
Catalog No.:ELI-52896m
Gene Name:Mouse Sprr3
Detect Range:0.156-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse Sprr3
Alternative Name:Mouse Small proline-rich protein 3, Sprr3
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Small proline- rich protein 3, Sprr3 ELISA KIT allows for the in vitro quantitative determination of Mouse Sprr3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Small proline- rich protein 3, Sprr3 ELISA KIT has been pre-coated with an Mouse Small proline-rich protein 3, Sprr3 antibody specific to Mouse Sprr3 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Sprr3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Sprr3 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Sprr3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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: 3128136151
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- 作者
- 内容
- 询问日期
文献和实验玉米Proline responding 1(pro1)突变在蛋白合成和细胞周期调控中起到关键作用
上海大学生命科学学院、上海市能源作物育种及应用重点实验室的研究人员证实,玉米Pro1基因(Zm P5CS2)的突变造成了突变体细胞中脯氨酸(proline)合成受阻,从而导致proline积累的减少。突变体中proline的缺乏引起了相应的转运RNA(tRNApro AGG)空载形式(uncharged tRNApro AGG)的增多,从而激活了细胞中的GCN2激酶,该激酶通过磷酸化真核生物翻译起始因子eIF2α从而抑制了细胞中蛋白合成的普遍抑制;另一方面,突变体中proline的缺乏也引起
Primary Mouse Small Intestinal Epithelial Cell Cultures
The intestinal epithelium is the most rapidly self-renewing tissue in adult mammals. We have recently shown that Lgr5 (Leucine-rich repeat-containing G protein-coupled receptor) is expressed in intestinal stem cells by an in vivo genetic
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
stock solution Nutrient-rich fly medium Phosphate-buffered saline (PBS; pH 7.5) Poly-L-lysine solution (0.1% [w/v] in H2 O; Sigma P8920) Sodium phosphate buffer (10 mM, pH 6.8) Triton X-100 VECTASTAIN Elite ABC Kit
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