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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
RING finger protein 150, Rnf150
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse RING finger protein 150, Rnf150 ELISA KIT
Product Name:Mouse RING finger protein 150, Rnf150 ELISA KIT
Packing:96T
Catalog No.:ELI-41387m
Gene Name:Mouse Rnf150
Detect Range:78-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse Rnf150
Alternative Name:Mouse RING finger protein 150, Rnf150
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse RING finger protein 150, Rnf150 ELISA KIT allows for the in vitro quantitative determination of Mouse Rnf150 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse RING finger protein 150, Rnf150 ELISA KIT has been pre-coated with an Mouse RING finger protein 150, Rnf150 antibody specific to Mouse Rnf150 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Rnf150 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Rnf150 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Rnf150 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Mapping Protein‐Protein Interactions with Phage‐Displayed Combinatorial Peptide Libraries
by purification of selected phage by ELISA. Alternatively, there is a bead?based affinity selection method. These methods allow one to readily isolate peptide ligands that bind to a protein target of interest and use the consensus sequence to search proteomic
Studies of the Ubiquitin Proteasome System
and Translation Systems In Vitro Production of Ubiquitin‐Protein Ligases (E3s) Basic Protocol 24: Expression of Ring‐Finger E3s in Bacterial Lysates Alternate Protocol 14: Expression of HECT and U‐Box E3s in Bacterial Lysates Alternate Protocol 15: Expression
文献速递|Cell 封面:应激颗粒异常互作介导 CMT2 周围神经病变的共同机制
., Aberrant oligodendroglial-vascular interactions disrupt the blood-brain barrier, triggering CNS inflammation. Nat Neurosci 22, 709-718 (2019). 3.J. Niu et al., Oligodendroglial ring finger protein Rnf43 is an essential injury-specific regulator
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