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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Rapamycin-insensitive companion of mTOR, Rictor
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Rapamycin- insensitive companion of mTOR, Rictor ELISA KIT
Product Name:Mouse Rapamycin- insensitive companion of mTOR, Rictor ELISA KIT
Packing:96T
Catalog No.:ELI-41325m
Gene Name:Mouse Rictor
Detect Range:78.1-5000pg/ml
Sensitivity:46.875pg/ml
Target Protein Name:Mouse Rictor
Alternative Name:Mouse Rapamycin-insensitive companion of mTOR, Rictor
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Rapamycin- insensitive companion of mTOR, Rictor ELISA KIT allows for the in vitro quantitative determination of Mouse Rictor concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Rapamycin- insensitive companion of mTOR, Rictor ELISA KIT has been pre-coated with an Mouse Rapamycin-insensitive companion of mTOR, Rictor antibody specific to Mouse Rictor .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Rictor and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Rictor , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Rictor in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
:
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文献和实验Inducible raptor and rictor Knockout Mouse Embryonic Fibroblasts
The mammalian Target of Rapamycin (mTOR) kinase functions within two structurally and functionally distinct multiprotein complexes termed mTOR complex 1 (mTORC1) and mTORC2. The immunosuppressant and anticancer drug rapamycin is commonly used
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