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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
40S ribosomal protein S3a, Rps3a
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse 40S ribosomal protein S3a, RPS3A ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
RPS3A,FTE1; MFTL; S3A; 40S ribosomal protein S3a; fte-1; v-fos transformation effector protein 1; ribosomal protein S3A
Search name
Mouse RPS3A ELISA KIT ,Mouse FTE1 ELISA KIT ,Mouse MFTL ELISA KIT ,Mouse S3A ELISA KIT ,Mouse 40S ribosomal protein S3a ELISA KIT ,Mouse fte-1 ELISA KIT ,Mouse v-fos transformation effector protein 1 ELISA KIT ,Mouse ribosomal protein S3A ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse 40S ribosomal protein S3a, RPS3A concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to RPS3A. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for RPS3A and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain RPS3A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of RPS3A in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验the binding sites for the 40S ribosomal subunit and for other translation initiation signals. Alternatively, the formation of a double-stranded DNA/RNA complex can render the RNA susceptible to RNase H digestion (2 ). The antigene approach is based
[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
acyltransferase, alpha) (AGPAT1), mRNA 742 NM_021134 Homo sapiens mitochondrial ribosomal protein L23 (MRPL23), mRNA 771 NM_021974 Homo sapiens polymerase (RNA) II (DNA directed) polypeptide F (POLR2F), mRNA 1169 NM_006808 Homo sapiens protein
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组在 Science 发表了题为 Cryo-EM structure of 90S small ribosomal subunit precursors in transition states 的论文,观察到酵母核糖体组装过程中小亚基从早期前体向晚期前体转化的一些列结构。一个来自德国的研究团队也在 Science 上发表了题为 90S pre-ribosome transformation into the primordial 40S subunit 的文章,该研究解释了 90S 核糖体前体 A1 位点的切割
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