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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白DNA excision repair protein ERCC-1, Ercc1含量
- 适应物种:
小鼠
- 标记物:
DNA excision repair protein ERCC-1, Ercc1
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse DNA excision repair protein ERCC-1, Ercc1 ELISA试剂盒
Product Name: Mouse DNA excision repair protein ERCC-1, Ercc1 ELISA试剂盒
Product Name: Mouse DNA excision repair protein ERCC-1, Ercc1 ELISA试剂盒
Gene Name: Mouse DNA excision repair protein ERCC-1, Ercc1
Detect Range:78-5000pg/ml
Sensitivity:39pg/ml
Target Protein Name: Mouse DNA excision repair protein ERCC-1, Ercc1
Alternative Name: Mouse DNA excision repair protein ERCC-1, Ercc1
ELISA type:Sandwich ELISA Kit
ELISA Test Principle:
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Product Description: Mouse DNA excision repair protein ERCC-1, Ercc1 ELISA试剂盒 allows for the in vitro quantitative determination of Mouse DNA excision repair protein ERCC-1, Ercc1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
The microtiter plate provided in Mouse DNA excision repair protein ERCC-1, Ercc1 ELISA试剂盒 has been pre-coated with an Mouse DNA excision repair protein ERCC-1, Ercc1 antibody specific to Mouse DNA excision repair protein ERCC-1, Ercc1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse DNA excision repair protein ERCC-1, Ercc1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse DNA excision repair protein ERCC-1, Ercc1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse DNA excision repair protein ERCC-1, Ercc1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Mammalian cells remove carcinogenic damage caused to DNA by ultraviolet (UV) light and certain other mutagens mainly by using the pathway known as nucleotide excision repair (NER). This involves damage recognition, unwinding of the DNA
In Vitro Excision Repair Assay in Schizosaccharomyces pombe
The measurement of DNA excision repair activity in vitro, originally developed by Wood and co-workers (1 ), utilizes transcriptionally active protein extracts obtained from mammalian cells by the method of Manley et al. (2 ) (see Chapter 29
In Vitro Monitoring of Base Excision Repair in Saccharomyces cerevisiae
of base damage and of individual mechanistic steps within BER. We used these protocols to investigate a new role of the DNA double strand break repair protein XRS1 in BER (1).
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