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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Ubiquitin-40S ribosomal protein S27a, Rps27a
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Ubiquitin- 40S ribosomal protein S27a, Rps27a ELISA KIT
Product Name:Mouse Ubiquitin- 40S ribosomal protein S27a, Rps27a ELISA KIT
Packing:96T
Catalog No.:ELI-38700m
Gene Name:Mouse Rps27a
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Rps27a
Alternative Name:Mouse Ubiquitin-40S ribosomal protein S27a, Rps27a
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Ubiquitin- 40S ribosomal protein S27a, Rps27a ELISA KIT allows for the in vitro quantitative determination of Mouse Rps27a concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Ubiquitin- 40S ribosomal protein S27a, Rps27a ELISA KIT has been pre-coated with an Mouse Ubiquitin-40S ribosomal protein S27a, Rps27a antibody specific to Mouse Rps27a .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Rps27a and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Rps27a , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Rps27a in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Studies of the Ubiquitin Proteasome System
studied outcome of ubiquitylation, we now understand that modification of proteins with ubiquitin has numerous other cellular roles that alter protein function and that are unrelated to proteasomal degradation. Ubiquitylation is a complex process
Membrane‐Based Yeast Two‐Hybrid System to Detect Protein Interactions
2H) system presented in this unit overcomes some of these limitations. It is based on the split?ubiquitin protein complementation assay and detects protein interactions directly at the membrane, thereby allowing the use of full?length integral
[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
(GAPD), mRNA 828 *NM_000291 Homo sapiens phosphoglycerate kinase 1 (PGK1), mRNA 2727 *NM_005566 Homo sapiens lactate dehydrogenase A (LDHA), mRNA 2105 *NM_002954 Homo sapiens ribosomal protein S27a (RPS27A), mRNA 4156 *NM_000981 Homo sapiens
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