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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Ras-related GTP-binding protein D, Rragd
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Ras- related GTP- binding protein D, Rragd ELISA KIT
Product Name:Mouse Ras- related GTP- binding protein D, Rragd ELISA KIT
Packing:96T
Catalog No.:ELI-38687m
Gene Name:Mouse Rragd
Detect Range:0.156-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse Rragd
Alternative Name:Mouse Ras-related GTP-binding protein D, Rragd
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Ras- related GTP- binding protein D, Rragd ELISA KIT allows for the in vitro quantitative determination of Mouse Rragd concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Ras- related GTP- binding protein D, Rragd ELISA KIT has been pre-coated with an Mouse Ras-related GTP-binding protein D, Rragd antibody specific to Mouse Rragd .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Rragd and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Rragd , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Rragd in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
:
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文献和实验, which are active in the GTP-bound form and inactive in the GDP-bound form. Given the importance of studies on the Ras protein activation state to understand the detailed mechanism of Ras-mediated signal transduction, we provide here a simple
小GTP结合蛋白(small GTP binding protein)
), 它催化GDT同GTP的交换。另一种是GTP 酶激活蛋白(GTPase-activating protein, GAP), 它触发结合的GTP水解。 许多小GTP结合蛋白都有一个共价结合的脂基团, 帮助它们同膜结合。小GTP蛋白包括ras、rab和rho蛋白, 它们在一些重要的的活动中起调节作用, 包括分泌活动和细胞骨架的装配等。
Nonradioactive Methods for Detecting Activation of Ras-Related Small G Proteins
active form of Ras -related G proteins involved immunoprecipitation of 32 P-labeled protein followed by separation of the labeled GTP/GDP bound to GTPase. A new method based on the large affinity difference of the GTP- and GDP-bound form of Ras proteins
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