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- 详细信息
- 文献和实验
- 技术资料
- 库存:
10
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Type I iodothyronine deiodinase, Dio1
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Type I iodothyronine deiodinase, DIO1 ELISA KIT
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
DIO1,5DI; TXDI1; DIOI; thyroxine deiodinase type I selenoprotein; type 1 DI; type I iodothyronine deiodinase; type-I 5'-deiodinase; deiodinase iodothyronine, type I ,Type I iodothyronine deiodinase,Type-I 5'-deiodinase,Type 1 DI,5DI,DIOI,
Search name
Mouse DIO1 ELISA KIT ,Mouse 5DI ELISA KIT ,Mouse TXDI1 ELISA KIT ,Mouse DIOI ELISA KIT ,Mouse thyroxine deiodinase type I selenoprotein ELISA KIT ,Mouse type 1 DI ELISA KIT ,Mouse type I iodothyronine deiodinase ELISA KIT ,Mouse type-I 5'-deiodinase ELISA KIT ,Mouse deiodinase iodothyronine type I ELISA KIT ,Mouse Type I iodothyronine deiodinase ELISA KIT ,Mouse Type-I 5'-deiodinase ELISA KIT ,Mouse Type 1 DI ELISA KIT ,Mouse 5DI ELISA KIT ,Mouse DIOI ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse type Type I iodothyronine deiodinase concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Type I iodothyronine deiodinase, During the reaction, Type I iodothyronine deiodinase in the sample or standard competes with a fixed amount of biotin-labeled Type I iodothyronine deiodinase for sites on a pre-coated Monoclonal antibody specific to Type I iodothyronine deiodinase. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Type I iodothyronine deiodinase in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
Bone Mineral Content and Density
Bassett, J.H., Boyde, A., Howell, P.G., Bassett, R.H., Galliford, T.M., Archanco, M., Evans, H., Lawson, M.A., Croucher, P., St Germain, D.L., Galton, V.A., and Williams, G.R. 2010. Optimal bone strength and mineralization requires the type 2 iodothyronine
特征、生物学标志或功能的纯化细胞群。细胞株常用于特定的研究中,例如,某些细胞株被用于生产蛋白质、疫苗或其他生物制品。 Section.02 实验细胞:培养与污染 很多人不太看重培养条件,认为自家细胞“能活”就行。但实际上,培养条件本身就会影响实验结果。 ——大家购买细胞时要看下培养说明,使用合适的细胞培养基培养。如在 ATCC ( American Type Culture Collection) 购买的细胞系来源和培养条件都可在其官网查询得到。当然,往期也有为大家做过常用实验细胞
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