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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Histone-lysine N-methyltransferase MLL2, Mll2
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Histone- lysine N- methyltransferase MLL2, Mll2 ELISA KIT
Product Name:Mouse Histone- lysine N- methyltransferase MLL2, Mll2 ELISA KIT
Packing:96T
Catalog No.:ELI-37271m
Gene Name:Mouse Mll2
Detect Range:0.15-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse Mll2
Alternative Name:Mouse Histone-lysine N-methyltransferase MLL2, Mll2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Histone- lysine N- methyltransferase MLL2, Mll2 ELISA KIT allows for the in vitro quantitative determination of Mouse Mll2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Histone- lysine N- methyltransferase MLL2, Mll2 ELISA KIT has been pre-coated with an Mouse Histone-lysine N-methyltransferase MLL2, Mll2 antibody specific to Mouse Mll2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Mll2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Mll2 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Mll2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验(50) - Menzel Gläser (VWR 8032-E01) Triton®-X-100 - ICN 807423 Tween20 - Sigma® P9416 Vanadyl Ribonucleoside Complex (VRC) - N. E. Biolabs® S1402S Nick Translation Kit - VYSIS® (Abbott 32-801300) Water 500ml - Sigma® W3500 [1]
Performing Bronchoalveolar Lavage in the Mouse
lavage. Vet. Clin. Small Anim. 33:69‐88. Blé, F.‐X, Cannet, C., Zurbruegg, S., Karmouty Quintana, H., Frossard, N., Trifilieff, A., and Beckmann, N. 2008. Allergen‐induced lung
of precipitated chromatin are provided in Noma et al. (2001) and Gregory et al. (2001).Figure 1. Allele-specific patterns of histone modifications revealed by PCR amplification and SSCP electrophoresis. Lung tissue was dissected from a mouse
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