Intended use This immunoassay kit allows for the in vitro quantitative determination ofmouseHistone H4concentrations in serum, Plasma,tissue homogenates and Cell culture supernates and Other biological fluids. Test principle The microtiter plate provided in this kit has been pre-coated with an antibody specific to Histone H4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific forHistone H4 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Histone H4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Histone H4 in the samples is then determined by comparing the O.D. of the samples to the standard curve. 产品详细信息请点击:Mouse Histone H4 ELISA Kit
et al,CYLD deficiency exacerbates lipopolysaccharide (LPS)-Induced pyroptosis in astrocytes of mice with sepsis. In Biochem Biophys Res Commun on 2019 Jul,by Li L, Shu MQ, et al..PMID:31097224,, (2019), PMID: 31097224