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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Centrosomal protein of 290 kDa, Cep290
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Centrosomal protein of 290 kDa, Cep290 ELISA KIT
Product Name:Mouse Centrosomal protein of 290 kDa, Cep290 ELISA KIT
Packing:96T
Catalog No.:ELI-34194m
Gene Name:Mouse Cep290
Detect Range:0.156-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse Cep290
Alternative Name:Mouse Centrosomal protein of 290 kDa, Cep290
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Centrosomal protein of 290 kDa, Cep290 ELISA KIT allows for the in vitro quantitative determination of Mouse Cep290 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Centrosomal protein of 290 kDa, Cep290 ELISA KIT has been pre-coated with an Mouse Centrosomal protein of 290 kDa, Cep290 antibody specific to Mouse Cep290 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Cep290 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Cep290 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Cep290 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
Discovery Genetics: The History and Future of Spontaneous Mutation Research
.S., Heckenlively, J.R., and Swaroop, A. 2006. In‐frame deletion in a novel centrosomal/ciliary protein CEP290/NPHP6 perturbs its interaction with RPGR and results in early‐onset retinal degeneration in the rd16 mouse. Hum. Mol. Genet. 15:1847‐1857
High‐Throughput, Multiplexed Analysis of 3‐Nitrotyrosine in Individual Proteins
Daly, D.S., Anderson, K.K., White, A.M., Gonzalez, R.M., Varnum, S.M., and Zangar, R.C. 2008. Predicting protein concentrations with ELISA microarray assays, monotonic splines and Monte Carlo simulation. Stat. Appl. Genet. Mol. Biol. 7: Article21
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