- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-33267m
- 2025年07月10日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
C1QTNF1
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Complement C1q tumor necrosis factor-related protein 1, C1QTNF1 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
C1QTNF1,CTRP1; GIP; ZSIG37; G protein coupled receptor interacting protein; complement C1q tumor necrosis factor-related protein 1; g protein-coupled receptor-interacting protein; C1q and tumor necrosis factor related protein 1, Putative secreted protein ZSIG37
Search name
Mouse C1QTNF1 ELISA KIT ,Mouse CTRP1 ELISA KIT ,Mouse GIP ELISA KIT ,Mouse ZSIG37 ELISA KIT ,Mouse G protein coupled receptor interacting protein ELISA KIT ,Mouse complement C1q tumor necrosis factor-related protein 1 ELISA KIT ,Mouse g protein-coupled receptor-interacting protein ELISA KIT ,Mouse C1q and tumor necrosis factor related protein 1 ELISA KIT,Mouse Putative secreted protein ZSIG37 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of mouse complement c1q tumor necrosis factor-related protein 1,C1QTNF1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to C1QTNF1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for C1QTNF1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain C1QTNF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of C1QTNF1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF Manual
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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
, dry 5. block half of the plate (with platelets) by PBS-BSA 1% and coat another half by glycine (x1) - BSA for 1 hour at 37ѓC 6. wash 3 times in PBS-tween 20, dry 7. incubated at 37ѓc for 1 hour with a 1:1000 dilution of rat IgG2a anti-mouse kappa
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