- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-32823m
- 2025年07月07日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
DNA ligase 1, Lig1
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse DNA ligase 1, Lig1 ELISA KIT
Product Name:Mouse DNA ligase 1, Lig1 ELISA KIT
Packing:96T
Catalog No.:ELI-32823m
Gene Name:Mouse Lig1
Detect Range:78-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse Lig1
Alternative Name:Mouse DNA ligase 1, Lig1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse DNA ligase 1, Lig1 ELISA KIT allows for the in vitro quantitative determination of Mouse Lig1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse DNA ligase 1, Lig1 ELISA KIT has been pre-coated with an Mouse DNA ligase 1, Lig1 antibody specific to Mouse Lig1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Lig1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Lig1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Lig1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验resulting from real-time PCR detection of the E6 and E7 oncogenes is discussed. Other methods for viral nucleic acid detection, including nested PCR amplification, ligase chain reaction, and enzyme-linked immunosorbent assay (ELISA), are also briefly
1ul 10mM rATP 1 ul T4 DNA Ligase(4U/ul) 3. 混匀后,4℃连接3days,或者8℃过夜连接;六、双链 cDNA 末端的磷酸化及 Xho I 酶切 : 1. 连接反应完成后,将反应体系70℃放置15分钟灭活T4 DNA Ligase; 2. 稍微离心使反应物集中至管底,室温下放置5分钟,然后加入下列试剂: 1ul 10×Ligase Buffer 1ul 10mM rATP 6ul dd H2 O
PCR 的装置。 4、杂交:PCR 扩增结束后，用标记的寡核苷酸探针进行原位杂交。 5、显微镜观察结果。 原位 PCR 既能分辩鉴定带有靶序列的细胞，又能标出靶序列在细胞内的位置，于分子和细胞水平上研究疾病的发病机理和临床过程及病理的转归有重大的实用价值。其特异性和敏感性高于一般的 PCR。 八、连接酶链反应(Ligase chain reaction，LCR) 连接酶链反应(Ligase chain reaction，LCR)，是一种新的 DNA 体外扩增和检测技术，主要用于点突变的研究及靶基因
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