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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Glycerol kinase, Gk
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Glycerol kinase, Gk ELISA KIT
Product Name:Mouse Glycerol kinase, Gk ELISA KIT
Packing:96T
Catalog No.:ELI-32684m
Gene Name:Mouse Gk
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Gk
Alternative Name:Mouse Glycerol kinase, Gk
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Glycerol kinase, Gk ELISA KIT allows for the in vitro quantitative determination of Mouse Gk concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Glycerol kinase, Gk ELISA KIT has been pre-coated with an Mouse Glycerol kinase, Gk antibody specific to Mouse Gk .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Gk and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Gk , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Gk in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Identification of a Mutant Kinase/ATP Analog Pair
M2 Lysis buffer (cold) [II], freshly prepared 50 mM Tris-base (pH 7.4) 150 mM NaCl 10% glycerol 1% Triton X-100 0.5 mM EDTA 0.5 mM EGTA 50 mM NaF
Identification of a Mutant Kinase/ATP Analog Pair
M2 Lysis buffer (cold) [II], freshly prepared 50 mM Tris-base (pH 7.4) 150 mM NaCl 10% glycerol 1% Triton X-100 0.5 mM EDTA 0.5 mM EGTA 50 mM NaF
Preparation of Recombinant Protein Spotted Arrays for Proteome‐Wide Identification of Kinase Targets
microarrays per kinase to be probed (e.g., from protocol 2 ) Superblock/0.1% Triton X‐100 (Thermo/Fisher) Kinase to be assayed
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