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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Glycerol kinase 2, Gk2
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Glycerol kinase 2, Gk2 ELISA KIT
Product Name:Mouse Glycerol kinase 2, Gk2 ELISA KIT
Packing:96T
Catalog No.:ELI-27990m
Gene Name:Mouse Gk2
Detect Range:0.156-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse Gk2
Alternative Name:Mouse Glycerol kinase 2, Gk2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Glycerol kinase 2, Gk2 ELISA KIT allows for the in vitro quantitative determination of Mouse Gk2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Glycerol kinase 2, Gk2 ELISA KIT has been pre-coated with an Mouse Glycerol kinase 2, Gk2 antibody specific to Mouse Gk2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Gk2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Gk2 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Gk2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Proteins on Glass Slides. Proteins were dissolved in 40% glycerol, 60% PBS, pH 7.5 at a concentration of 100 μg/ml unless indicated otherwise. For Figs. 1, 5, 6, and 7, the proteins were spotted on aldehyde slides using a GMS 417 Arrayer (Affymetrix
Studies of the Ubiquitin Proteasome System
and Purification of Anti‐Ubiquitin Antibodies Support Protocol 3: Preparation of Ubiquitin Affinity Column Generation of Reagent‐Grade Ubiquitin Activating Enzyme (E1) Basic Protocol 20: Preparation of Wheat E1 in E. coli Basic Protocol 21: Preparation of Mouse E1
Human Complement Components C4A and C4B Genetic Diversities: Complex Genotypes and Phenotypes
Platinum Taq PCR x DNA Polymerase (Invitrogen; or equivalent commercial high‐fidelity Taq polymerase kit), including: 10× PCR buffer
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