ARF GTPase-activating protein
GIT2, Git2 ELISA KIT/ Mouse ARF GTPase-activating protein GIT2, Git2 ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-32675m
- 2025年07月08日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
ARF GTPase-activating protein GIT2, Git2
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse ARF GTPase- activating protein GIT2, Git2 ELISA KIT
Product Name:Mouse ARF GTPase- activating protein GIT2, Git2 ELISA KIT
Packing:96T
Catalog No.:ELI-32675m
Gene Name:Mouse Git2
Detect Range:0.156-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse Git2
Alternative Name:Mouse ARF GTPase-activating protein GIT2, Git2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse ARF GTPase- activating protein GIT2, Git2 ELISA KIT allows for the in vitro quantitative determination of Mouse Git2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse ARF GTPase- activating protein GIT2, Git2 ELISA KIT has been pre-coated with an Mouse ARF GTPase-activating protein GIT2, Git2 antibody specific to Mouse Git2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Git2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Git2 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Git2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验ARF GTPase-Activating Protein 1
of intrinsic GTPase activity, and GTP hydrolysis depends on the action of GTPase-activating proteins (GAPs). ARF GAPs are a family of proteins which contain a conserved GAP domain of approx 130 amino acids with a unique Cys4 zinc-finger motif near their amino
ARF-Directed Guanine-Nucleotide-Exchange (GEP) Proteins
5-kinase (8 ). ARF, like other regulatory GTPases, is active when GTP is bound. The inactivation of ARF-GTP requires its interaction with a GTPase-activating protein (GAP), which accelerates GTP hydrolysis. Activation of the inactive GDP-bound ARF requires
装配反应因子(assembly reaction factor, ARF)
一类与被膜小泡装配相关的蛋白因子。在COP被膜小泡装配中, ARF被认为是外被体外被的装配和去装配的信号。装配反应因子是一种单体GTPase，由一条多肽链组成，同时含有一个脂肪酸的尾部，属单体GTP结合蛋白(monomeric GTP-binding protein)。 ARF在COP-被膜小泡形成的过程中起重要作用。当ARF同GDP结合时，没有活性，其脂肪酸的尾部隐藏在蛋白质的空间结构中，ARF也就不能同膜结合。当无活性的ARF�GDP同供体膜上的鸟
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