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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Tyrosine-protein kinase JAK1, Jak1
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Tyrosine- protein kinase JAK1, Jak1 ELISA KIT
Product Name:Mouse Tyrosine- protein kinase JAK1, Jak1 ELISA KIT
Packing:96T
Catalog No.:ELI-38161m
Gene Name:Mouse Jak1
Detect Range:78.125-5000pg/ml
Sensitivity:46.875pg/ml
Target Protein Name:Mouse Jak1
Alternative Name:Mouse Tyrosine-protein kinase JAK1, Jak1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Tyrosine- protein kinase JAK1, Jak1 ELISA KIT allows for the in vitro quantitative determination of Mouse Jak1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Tyrosine- protein kinase JAK1, Jak1 ELISA KIT has been pre-coated with an Mouse Tyrosine-protein kinase JAK1, Jak1 antibody specific to Mouse Jak1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Jak1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Jak1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Jak1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Visualization of Protein Kinase Activities in Living Cells
organisms ranging from Caenorhabditis elegans to mouse, permitting visualization of spatio-temporal regulation of kinase activities and systemic understanding of the signaling networks in living animals.
Translocation Assays of Protein Kinase C Activation
The protein kinase C (PKC) family members include at least 11 different isoforms that, based on their different requirements for activation, have been divided into three subfamilies, the Ca2+ -dependent (cPKCα, (βI , βII
Protein Techniques: Immunoprecipitation, In Vitro Kinase Assays, and Western Blotting
. For example, the introduction of a portion of the Y chromosome into a female mouse can change its sex, demonstrating the presence of a sex-determining gene (1 ). In combination with a range of molecular biology techniques, such as RNA protection, PCR
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