Dynein heavy chain 12, axonema
l, Dnah12 ELISA KIT/ Mouse Dynein heavy chain 12, axonemal, Dnah12 ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-32294m
- 2025年07月10日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Dynein heavy chain 12, axonemal, Dnah12
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Dynein heavy chain 12, axonemal, Dnah12 ELISA KIT
Product Name:Mouse Dynein heavy chain 12, axonemal, Dnah12 ELISA KIT
Packing:96T
Catalog No.:ELI-32294m
Gene Name:Mouse Dnah12
Detect Range:78.125-5000pg/ml
Sensitivity:46.875pg/ml
Target Protein Name:Mouse Dnah12
Alternative Name:Mouse Dynein heavy chain 12, axonemal, Dnah12
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Dynein heavy chain 12, axonemal, Dnah12 ELISA KIT allows for the in vitro quantitative determination of Mouse Dnah12 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Dynein heavy chain 12, axonemal, Dnah12 ELISA KIT has been pre-coated with an Mouse Dynein heavy chain 12, axonemal, Dnah12 antibody specific to Mouse Dnah12 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Dnah12 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Dnah12 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Dnah12 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Evaluating the Dynein Heavy Chain Gene Family in Tetrahymena
In this chapter, we describe our methods to evaluate the dynein heavy chain genes expressed in a cell. Dynein is the high molecular weight molecular motor that produces directed movement along microtubules. All known dyneins travel
Manipulating Dynein Genes in Tetrahymena
In this chapter, we outline methods to modify targeted genes in Tetrahymena . Such a strategy could be utilized to deduce the function of individual dynein isoforms, as well as to dissect the functional domains within a single heavy chain.
Rapid Isotyping of Mouse Monoclonal Antibodies
for 5% of mouse antibodies) has limited practical value. In contrast, the heavy-chain isotype is very informative (see Table 1 ); protein A or protein G binding, euglobulin properties, complement binding, kinetics of digestion with papain or pepsin, clearance
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