Dual specificity protein phosp

Mouse Dual specificity protein phosphatase 10, DUSP10 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Dusp10,MKP-5; MKP5; dual specificity phosphatase MKP-5; dual specificity protein phosphatase 10; map kinase phosphatase 5; mitogen-activated protein kinase phosphatase 5; serine/threonine specific protein phosphatase; dual specificity phosphatase 10

Search name

Mouse Dusp10 ELISA KIT ,Mouse MKP-5 ELISA KIT ,Mouse MKP5 ELISA KIT ,Mouse dual specificity phosphatase MKP-5 ELISA KIT ,Mouse dual specificity protein phosphatase 10 ELISA KIT ,Mouse map kinase phosphatase 5 ELISA KIT ,Mouse mitogen-activated protein kinase phosphatase 5 ELISA KIT ,Mouse serine/threonine specific protein phosphatase ELISA KIT ,Mouse dual specificity phosphatase 10 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Mouse Dual specificity protein phosphatase 10, DUSP10 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to DUSP10. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for DUSP10 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain DUSP10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of DUSP10 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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