- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-32284m
- 2026年05月21日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
10
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Dual specificity protein phosphatase 10, Dusp10
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Dual specificity protein phosphatase 10, DUSP10 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Dusp10,MKP-5; MKP5; dual specificity phosphatase MKP-5; dual specificity protein phosphatase 10; map kinase phosphatase 5; mitogen-activated protein kinase phosphatase 5; serine/threonine specific protein phosphatase; dual specificity phosphatase 10
Search name
Mouse Dusp10 ELISA KIT ,Mouse MKP-5 ELISA KIT ,Mouse MKP5 ELISA KIT ,Mouse dual specificity phosphatase MKP-5 ELISA KIT ,Mouse dual specificity protein phosphatase 10 ELISA KIT ,Mouse map kinase phosphatase 5 ELISA KIT ,Mouse mitogen-activated protein kinase phosphatase 5 ELISA KIT ,Mouse serine/threonine specific protein phosphatase ELISA KIT ,Mouse dual specificity phosphatase 10 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse Dual specificity protein phosphatase 10, DUSP10 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to DUSP10. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for DUSP10 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain DUSP10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of DUSP10 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Dual- and Triple-Color Fluorospot
in the analysis, e.g., IL-10, rat mAb 9D7 is used for coating and tag (different from FITC and biotin)-labeled rat mAb 12G8 is used for detection. All mAbs from Mabtech, Nacka Strand, Sweden. 4. Secondary reagents for amplification: Mouse anti-FITC mAb
ELISA原理与实验方法The advantages of the ELISA are similar to other antibody-labeled reactions which include specificity, sensitivity, inexpensiveness, and safety. Since the enzyme label is the critical portion of ELISA, its selection is very important
Specificity Profiling of Protein‐Binding Domains Using One‐Bead‐One‐Compound Peptide Libraries
, L., Kim, S., Carpino, N., Daniel, J.L., Kunapuli, S.P., Tsygankov, A.Y., and Pei, D.H. 2010. Determination of the substrate specificity of protein‐tyrosine phosphatase TULA‐2 and identification of Syk as a TULA‐2 substrate. J. Biol. Chem. 285:31268
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