Diacylglycerol kinase gamma, D
gkg ELISA KIT/ Mouse Diacylglycerol kinase gamma, Dgkg ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-32152m
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Diacylglycerol kinase gamma, Dgkg
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Diacylglycerol kinase gamma, Dgkg ELISA KIT
Product Name:Mouse Diacylglycerol kinase gamma, Dgkg ELISA KIT
Packing:96T
Catalog No.:ELI-32152m
Gene Name:Mouse Dgkg
Detect Range:78.1-5000pg/ml
Sensitivity:46.875pg/ml
Target Protein Name:Mouse Dgkg
Alternative Name:Mouse Diacylglycerol kinase gamma, Dgkg
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Diacylglycerol kinase gamma, Dgkg ELISA KIT allows for the in vitro quantitative determination of Mouse Dgkg concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Diacylglycerol kinase gamma, Dgkg ELISA KIT has been pre-coated with an Mouse Diacylglycerol kinase gamma, Dgkg antibody specific to Mouse Dgkg .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Dgkg and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Dgkg , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Dgkg in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Phosphoinositides, Inositol Phosphates, and Phospholipase C in Embryonic Stem Cells
is involved in the release of intracellu-lar calcium from internal stores, whereas the lipophilic diacylglycerol is involved in protein kinase C activation. Inositol supplementation is essential for the in vitro growth of rabbit blastocysts, and studies
with the recommended amount of ATP, 2 μl of gamma-33P-ATP (20 μCi), and 2 μl of purified enzyme (10 units of cAMP-dependent protein kinase (catalytic subunit), 1000 units of casein kinase II, or 100 units of Erk2). Following the 1 hr incubation, the slides were washed
HIGH RESOLUTION GENETIC FOOTPRINTING
gamma-32P-ATP (100 uCi) 10 ul 10X kinase buffer 5 ul T4 polynucleotide kinase (50 U) [New England Biolabs] 74 ul water Incubate 37 C for 1 hr. Incubate 65 C for 20 min to heat inactivate kinase. Run over a G-15 Sephadex spin column to get rid
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